Characterization of the T-lymphocyte response elicited by mouse immunization with rat platelets.
Exp Hematol
; 39(6): 676-85, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21397657
ABSTRACT
OBJECTIVE:
Immunization of normal CBA mice with rat platelets leads to an autoantibody response directed against mouse platelets. The purpose of this work was to determine the involvement of T lymphocytes in this response. MATERIALS ANDMETHODS:
T-lymphocyte responses were analyzed in vivo by depletion and transfer experiments and ex vivo by proliferation assay and cytokine measurements.RESULTS:
Mouse immunization with rat platelets induced production of antibodies reacting with rat and mouse platelets. This response was found to depend on CD4(+) T-helper lymphocytes reacting with rat, but not with mouse platelets. These anti-rat platelet T-helper cells were mainly of the Th1 phenotype. When transferred into naïve mice, they enhanced the anti-mouse platelet antibody response induced by subsequent immunization with rat platelets. In addition, depletion of CD25(+) cells enhanced the thrombocytopenia induced by immunization with rat platelets, whereas adoptive transfer of CD4(+)CD25(+) cells from immunized mice suppressed it.CONCLUSIONS:
Our results suggest that activation of anti-rat platelet T-helper cells can bypass the mechanism of tolerance and result in the secretion of autoreactive antibodies, but this response is still controlled by regulatory T cells that develop progressively after immunization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Linfócitos T Auxiliares-Indutores
/
Transferência Adotiva
Limite:
Animals
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Bélgica