Your browser doesn't support javascript.
loading
In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia.
Ali, Intzar; Sharma, Punita; Suri, Krishan Avtar; Satti, Naresh Kumar; Dutt, Prabhu; Afrin, Farhat; Khan, Inshad Ali.
Afiliação
  • Ali I; Clinical Microbiology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
  • Sharma P; Natural Product Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
  • Suri KA; Natural Product Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
  • Satti NK; Natural Product Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
  • Dutt P; Natural Product Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
  • Afrin F; Department of Biotechnology, Faculty of Science, Hamdard University, Hamdard Nagar, New Delhi 110 062, India.
  • Khan IA; Clinical Microbiology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180 001, India.
J Med Microbiol ; 60(Pt 9): 1326-1336, 2011 Sep.
Article em En | MEDLINE | ID: mdl-21474610
This study was undertaken to investigate the synergistic interaction between amphotericin B (AmB) and acteoside, isolated from the aerial parts of the shrub Colebrookea oppositifolia (Lamiaceae). Acteoside alone exhibited no intrinsic antifungal activity but showed a potent synergism in combination with AmB against selected pathogenic species, with fractional inhibitory concentration indices in the range of 0.0312-0.1562. The combination of acteoside at 3.12 and 12.5 µg ml(-1) with subinhibitory concentrations of AmB resulted in a potent fungicidal effect and also exhibited a significantly extended post-antifungal effect. Furthermore, the combination also reduced the minimum biofilm reduction concentration values of AmB (2-16-fold) in preformed biofilms of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. There was decreased viability of the cells, increased uptake of propidium iodide and enhanced leakage of 260 nm-absorbing material by Candida albicans cells when exposed to AmB in the presence of acteoside. The reason for potentiation is likely to be that the subinhibitory concentrations of AmB facilitated the uptake of acteoside, which resulted in increased killing of the fungal cells. Administration of acteoside in mice at up to 2000 mg (kg body weight)(-1) by the intraperitoneal or oral route produced no overt toxicity. The data presented here support synergism between acteoside and AmB, and it is therefore proposed that a prospective new management strategy for therapeutic application of this combination should be explored.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenóis / Aspergillus fumigatus / Candida albicans / Anfotericina B / Cryptococcus neoformans / Sinergismo Farmacológico / Glucosídeos / Antifúngicos Limite: Animals Idioma: En Revista: J Med Microbiol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Índia País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenóis / Aspergillus fumigatus / Candida albicans / Anfotericina B / Cryptococcus neoformans / Sinergismo Farmacológico / Glucosídeos / Antifúngicos Limite: Animals Idioma: En Revista: J Med Microbiol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Índia País de publicação: Reino Unido