Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor.
Biotechnol Lett
; 33(8): 1565-73, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21476093
ABSTRACT
Embryoid body (EB) formation is a common intermediate during in vitro differentiation of pluripotent stem cells into specialized cell types. We have optimized the slow-turning, lateral vessel (STLV) for large scale and homogenous EB production from mouse embryonic stem cells. The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. Using 3 × 10(5) cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Técnicas de Cultura de Células
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Reatores Biológicos
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Miócitos Cardíacos
/
Células-Tronco Embrionárias
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Hungria