Polarimetric total internal reflection biosensing.
Opt Express
; 19(8): 7410-6, 2011 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-21503051
ABSTRACT
In this paper, a concept of polarimetric total internal reflection (TIR) biosensor based on the method of temporal phase modulation is presented. Measurements of the phase difference between s- and p- polarized light combined with their amplitudes allow simultaneous detection of the bulk refractive index and thickness of the surface biofilms. Obtained experimental sensitivity is better than 10(-5) in terms of refractive index unit and 0.5 nm in biolayer thickness. Relatively simple technological implementation of the TIR sensors on the base of inexpensive and transparent substrates opens a number of novel applications in biosensing and microscopy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Refratometria
/
Técnicas Biossensoriais
/
Óptica e Fotônica
/
Microscopia de Polarização
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Opt Express
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Canadá