Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea.
J Food Sci
; 75(8): H239-43, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-21535501
ABSTRACT
Gao-Cha is a traditional Chinese health tea made from Acer ginnala. We performed a components and radical scavenging activity analysis to identify any medicinal components in this tea. High performance thin layer chromatography (HPTLC)-1,1-Diphenyl-2-picryhydrazyl (HPTLC-DPPH) assay showed that the methanolic extract contained strong radical scavengers. Quantitative analysis revealed that the IC(50) of the extract against 1 mM DPPH was 52.7 ± 0.6 µg/mL. Bioactive-guided isolations led to procurement of 3 radical scavengers with IC(50)s of 17.5 ± 2.1, 29.3 ± 2.5, and 21.6 ± 1.7 µg/mL, respectively. Analysis of the high resolution-electric spray ionization-mass spectrometer and (1)H, (13)C, distortionless enhancement by polarization transfer at 135°, heteronuclear quantum coherence, correlating spectroscopy coupling, and heteronuclear multiple bond coherence (HMBC) data revealed that the compounds were methyl 3, 4, 5-trihydroxybenzoate (1), quercetin-3-O-α-rhamnopyranoside (2), and 2,6-bis (3,4,5-trihydroxybenzoyl)-aceritol (3). Bioactive combined components analysis revealed that, apart from compounds 1, 2, and 3, the tea possibly contained radical scavengers ginnalin A (4) and B (5), 2â³-O-Galloylquercitrin (6) and 3â³-O-Galloyl-quercitrin (7). Compounds 2, 6, and 7 were isolated from Acer ginnala for the first time. The positions of the 2 galloyl moieties in compound 3 were unambiguously established by the HMBC spectrum for the first time.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Sequestradores de Radicais Livres
/
Brotos de Planta
/
Folhas de Planta
/
Acer
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Food Sci
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China