Influence of cobalt and zinc exposure on mRNA expression profiles of metallothionein and cytocrome P450 in rainbow trout.
Biol Trace Elem Res
; 144(1-3): 781-9, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21547398
ABSTRACT
The present research aims to evaluate the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on metallothioneins and cytocrome P450. Mature rainbow trouts were exposed to 10 mg/L CoCl(2).6H(2)O and 1 mg/L ZnSO(4).7H(2)O. After 6, 12, 24, and 48 h of treatment, expressions of muscle MT-A, MT-B, and CYP P4501A1 mRNAs were measured by means of quantitative real-time polymerase chain reaction. During the exposure experiments, no mortalities occurred. We observed that expression levels of all genes increased with exposure time. Since the organism has not learned how to completely dispose of heavy metals and tends to bioaccumulate them, our results indicate that cobalt and zinc exposure may result in accumulation of the non-eliminated metals which may lead to fish death.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zinco
/
RNA Mensageiro
/
Cobalto
/
Oncorhynchus mykiss
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Sistema Enzimático do Citocromo P-450
/
Metalotioneína
Limite:
Animals
Idioma:
En
Revista:
Biol Trace Elem Res
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Turquia