Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)1.

ABSTRACT

PREMISE OF THE STUDY Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae). • METHODS AND RESULTS: Microsatellite loci were identified from genomic DNA sequences generated using the 454 GS-FLX titanium platform. Primers were designed for 67 tri- and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplification success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplified in individuals from across the species range. • CONCLUSIONS: These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis.