The heterologous systems in the study of cardosin B trafficking pathways.
Plant Signal Behav
; 6(6): 895-7, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21659795
ABSTRACT
Cardosins are abundant in cardoon pistils and were found to accumulate in different cell compartments cardosin A was detected in the vacuoles of stigmatic papillae and cardosin B accumulates in the extracellular matrix of the transmitting tissue. Due to the fact that cardoon system imposes some limitations to the study of processing and trafficking events, heterologous species have been employed to study cardosins trafficking pathways. Cardosin B was successfully expressed both in Arabidopsis and Tobacco plants, where it accumulated mainly in the vacuole but it was also detected in the cell wall. The glycosylation pattern of cardosin B was replicated in these systems - high-mannose type glycans. In tobacco leaves, cardosin B is transported through the Golgi in a RAB-D2a-dependent route, and is delivered to the vacuole via the prevacuolar compartment in a RAB-F2b-dependent pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Ácido Aspártico Endopeptidases
/
Plantas Geneticamente Modificadas
Idioma:
En
Revista:
Plant Signal Behav
Assunto da revista:
BOTANICA
/
FISIOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Portugal