A multiplexed immunoassay system based upon reciprocating centrifugal microfluidics.
Rev Sci Instrum
; 82(6): 064303, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21721711
ABSTRACT
A novel, centrifugal disk-based micro-total analysis system (µTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. The described reciprocating mechanism results in a reduction in processing time and reagent consumption by one order of magnitude.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Centrifugação
/
Técnicas Analíticas Microfluídicas
Limite:
Humans
Idioma:
En
Revista:
Rev Sci Instrum
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos