Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway.
J Cell Biol
; 194(3): 397-405, 2011 Aug 08.
Article
em En
| MEDLINE
| ID: mdl-21825074
ABSTRACT
The mitochondrial outer membrane (MOM) harbors several multispan proteins that execute various functions. Despite their importance, the mechanisms by which these proteins are recognized and inserted into the outer membrane remain largely unclear. In this paper, we address this issue using yeast mitochondria and the multispan protein Ugo1. Using a specific insertion assay and analysis by native gel electrophoresis, we show that the import receptor Tom70, but not its partner Tom20, is involved in the initial recognition of the Ugo1 precursor. Surprisingly, the import pore formed by the translocase of the outer membrane complex appears not to be required for the insertion process. Conversely, the multifunctional outer membrane protein mitochondrial import 1 (Mim1) plays a central role in mediating the insertion of Ugo1. Collectively, these results suggest that Ugo1 is inserted into the MOM by a novel pathway in which Tom70 and Mim1 contribute to the efficiency and selectivity of the process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas de Saccharomyces cerevisiae
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Proteínas Mitocondriais
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Proteínas de Transporte da Membrana Mitocondrial
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Proteínas de Membrana
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Alemanha