Comparison of three Chlamydomonas strains which show distinctive oxidative stress tolerance.
J Biosci Bioeng
; 112(5): 462-8, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21839677
ABSTRACT
Methyl viologen (MV) causes severe oxidative stress by generating superoxide in the photosystem. The marine Chlamydomonas strain W80 is highly tolerant to MV (inhibitory concentration 50% [IC50]=110 µM), and another marine Chlamydomonas strain HS5 shows also relatively a high tolerance (IC50=12 µM). These two marine strains and a freshwater Chlamydomonas reinhardtii, which is highly sensitive to MV (IC50=0.03 µM), were compared with respect to their reactive oxygen species (ROS) eliminating enzymes (superoxide dismutase, catalase, glutathione peroxidase, and ascorbate peroxidase), intracellular free amino acids, and antioxidant activities of the cell extracts. The marked difference between the marine Chlamydomonas strains and C. reinhardtii is the much higher (more than 5 fold) ascorbate peroxidase (APX) activity in the marine strains. The marine strains also kept the high APX activities (more than 100% of non-stressed condition) under the MV stressed condition, while the APX activity in C. reinhardtii was significantly decreased (36% of non-stressed condition) under the stressed condition, indicating that APX activity potentially contributes to the oxidative stress tolerance in Chlamydomonas. In addition, the levels of intracellular free proline, which is supposed to ameliorate oxidative stress, were several tens of times higher in the marine Chlamydomonas strains than in C. reinhardtii.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água do Mar
/
Chlamydomonas
/
Chlamydomonas reinhardtii
/
Água Doce
Limite:
Animals
Idioma:
En
Revista:
J Biosci Bioeng
Assunto da revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
JAPAN
/
JAPON
/
JAPÃO
/
JP