Expression of TLR 2, TLR 4 and iNOS in cervical monocytes of Chlamydia trachomatis-infected women and their role in host immune response.
Am J Reprod Immunol
; 66(6): 534-43, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21883620
ABSTRACT
PROBLEM:
To study the innate immune response -TLR2 TLR 4 and iNOS expression in female genital Chlamydia trachomatis infection.METHOD:
TLR 2, TLR 4, and iNOS expression was evaluated by real-time PCR in C. trachomatis-infected asymptomatic, mucopurulent cervicitis (MPC), and fertility disorders (FD) women. Expression of TLR signaling pathway genes was checked in vivo in C. trachomatis-infected cervical monocytes. Further, inos gene expression and nitric oxide release was assessed in vitro in THP-1 cell line upon chlamydial infection.RESULTS:
TLR2, TLR4, and iNOS expression was significantly (P < 0.05) higher in C. trachomatis-positive women with FD, MPC, and asymptomatic women, respectively, than in control. Chlamydial infection significantly upregulates CD86, TLR4, MyD88, IRAK2, nF-κB, IL-1,ß and IL-12 genes. Expression of iNOS gene was found to be significantly (P < 0.05) high 12 hrs post-infection.CONCLUSIONS:
Chlamydia trachomatis stimulates innate immune cells by activation of TLR2/TLR 4. Overall data indicate that recognition by TLR4 helps in initiation of immune response while recognition by TLR2 leads to secretion of inflammatory cytokines while iNOS-induced nitric oxide production helps in clearing Chlamydia. These results are first to provide initial insights into how innate immune response operates in human cervical monocytes upon chlamydial infection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
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Colo do Útero
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Chlamydia trachomatis
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Óxido Nítrico Sintase Tipo II
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Receptor 2 Toll-Like
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Receptor 4 Toll-Like
Limite:
Adult
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Female
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Humans
Idioma:
En
Revista:
Am J Reprod Immunol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Índia