Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25.
Science
; 248(4957): 866-8, 1990 May 18.
Article
em En
| MEDLINE
| ID: mdl-2188363
ABSTRACT
In Saccharomyces cerevisiae, the product of the CDC25 gene controls the RAS-mediated production of adenosine 3',5'-monophosphate (cAMP). In vivo the carboxyl-terminal third of the CDC25 gene product is sufficient for the activation of adenylate cyclase. The 3'-terminal part of SCD25, a gene of S. cerevisiae structurally related to CDC25, can suppress the requirement for CDC25. Partially purified preparations of the carboxy-terminal domain of the SCD25 gene product enhanced the exchange rate of guanosine diphosphate (GDP) to guanosine triphosphate (GTP) of pure RAS2 protein by stimulating the release of GDP. This protein fragment had a similar effect on the human c-H-ras-encoded p21 protein. Thus, the SCD25 carboxyl-terminal domain can enhance the regeneration of the active form of RAS proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas Fúngicas
/
Proteínas ras
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Proteínas de Ciclo Celular
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Ras-GRF1
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Proteínas de Saccharomyces cerevisiae
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Nucleotídeos de Guanina
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Guanosina Difosfato
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Guanosina Trifosfato
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
França