Immunohistochemical differentiation of inflammatory myopathies.
Neurol India
; 59(4): 513-20, 2011.
Article
em En
| MEDLINE
| ID: mdl-21891925
ABSTRACT
BACKGROUND:
Idiopathic inflammatory myopathies are a heterogeneous group of acquired muscle disorders with considerable overlap in the histological features, making histological diagnosis difficult at times.AIMS:
To determine the immunohistochemical profile of clinically suspected cases of inflammatory myopathies, using monoclonal antibodies to HLA-1 and membrane attack complex (MAC), and to correlate the clinical, serological, and electromyographic profile and the histopathological picture, with the immunohistochemical profile. SETTINGS ANDDESIGN:
This was a retrospective study analyzing the clinical and histopathological features in muscle of clinically suspected cases of inflammatory myopathy and correlating it to their HLA-1 and MAC immunostaining profiles. MATERIAL ANDMETHODS:
The study subjects included 33 cases with suspected inflammatory myopathy and 59 with non-inflammatory muscle disease, as controls. Clinical data, electromyographic findings, serological profile, and details of therapy were obtained from patient records. STATISTICALANALYSIS:
Student 'T' test, Pearson's Chi square test, and Kappa statistics were used appropriately.RESULTS:
Although HLA-1 and MAC immunostaining did not help to differentiate the individual subtypes of inflammatory myopathy, when either HLA-1 or MAC was positive, inflammatory myopathy could be ruled in with 86.5% certainty and when both HLA-1 and MAC were negative, it could be ruled out with 95% certainty.CONCLUSIONS:
A combination of clinical presentation, serological profile, electromyographic and histopathological features, together with the immunoprofile for HLA-1 and MAC, contribute toward making a diagnosis of inflammatory myopathy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
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Antígenos de Histocompatibilidade Classe I
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Complexo de Ataque à Membrana do Sistema Complemento
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Músculo Esquelético
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Miosite
Tipo de estudo:
Observational_studies
Limite:
Adolescent
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Adult
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Aged
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Middle aged
Idioma:
En
Revista:
Neurol India
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Índia