Segmentation of epithelium in H&E stained odontogenic cysts.
J Microsc
; 244(3): 273-92, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21974807
ABSTRACT
An algorithm for the automated segmentation of epithelial tissue in digital images of histologic tissue sections of odontogenic cysts (cysts originating from residual odontogenic epithelium) is presented. The algorithm features an image standardization process that greatly reduces variation in luminance and chrominance between images due to variations in sample preparation. Segmentation of the epithelial regions of images uses an algorithm based on binary graph cuts where graph weights depend on probabilities obtained from colour histogram models of epithelium and stroma image regions. Algorithm training used a data set of 38 images of four types of odontogenic cyst and was tested using a separate data set of 35 images of the same four cyst types. The best parameters for the segmentation algorithm were determined using a response-surface optimizer. The best parameter set resulted in an overall mean (± std. dev.) sensitivity of 91.5 ± 17% and overall mean specificity of 85.1 ± 18.6% on the training set. Particularly good results were obtained for dentigerous and odontogenic keratocysts for which the mean sensitivities/specificities were 91.9 ± 6.15%/97.4 ± 2.15% and 96.1 ± 1.98%/98.7 ± 3.16%, respectively. Our method is potentially applicable to many pathological conditions in similar tissues, such as skin and mucous membranes where there is a clear microscopic distinction between epithelium and connective tissues.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Patologia
/
Automação
/
Processamento de Imagem Assistida por Computador
/
Cistos Odontogênicos
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Epitélio
/
Histocitoquímica
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Microsc
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Canadá