Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran.
J Water Health
; 9(3): 603-8, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21976207
ABSTRACT
A comprehensive survey assessing the presence of Acanthamoeba was conducted on 50 samples from water sources in parks and public squares from 22 municipal districts of Tehran, Iran. The prevalence and genotypes of Acanthamoeba were determined by PCR and the PCR fragments of ribosomal RNA genes sequenced. Sixteen (32%) samples were positive for Acanthamoeba spp. Sequence analysis revealed that the positive isolates belonged to the T4 and T5 genotypes. Fourteen isolates (87.5%) were T4, and two (12.5%) were T5. Acanthamoeba may be a problematic organism for contact lens wearers and for immunocompromised individuals. In Iran, Acanthamoeba keratitis has increased in recent years, mainly due to poor hygiene in contact lens wearers. A thorough survey for the prevalence of this amoeba could have a significant role in prevention of disease. This is the first report of the T5 genotype from water in recreational areas of Tehran.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
Acanthamoeba
Tipo de estudo:
Risk_factors_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Water Health
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Irã
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM