Production of monoclonal antibody to herbicide fenoxaprop-ethyl.
Hybridoma (Larchmt)
; 30(5): 463-7, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-22008074
ABSTRACT
Fenoxaprop-ethyl is a selective aryloxyphenoxypropionate herbicide used widely to control annual and perennial grasses. A monoclonal antibody (MAb) against fenoxaprop-ethyl (FE), designated as 3E6B9C, was produced and had very low cross-reactivity with some of its structural analogs, such as clodinafop-propargyl, diclofop-methyl, lactofen, and quizalofop-p-ethyl. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed. The concentration of R-(+)-fenoxaprop-ethyl (R-FE) producing 50% of inhibition (IC(50)) and the working range of icELISA were 3.1 ng/mL and 0.6-29 ng/mL, respectively. This assay is also sensitive to R-fenoxaprop, S-(-)-fenoxaprop-ethyl, and metamifop with IC(50) of 3.4, 2.7, and 3.5 ng/mL, respectively. The recoveries of R-FE in soil samples with the icELISA were 86-102%.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxazóis
/
Propionatos
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Poluentes do Solo
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Ovalbumina
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Anticorpos Monoclonais Murinos
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Haptenos
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Herbicidas
Limite:
Animals
Idioma:
En
Revista:
Hybridoma (Larchmt)
Ano de publicação:
2011
Tipo de documento:
Article