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Genetic variation in the mouse model of Niemann Pick C1 affects female, as well as male, adiposity, and hepatic bile transporters but has indeterminate effects on caveolae.
Jelinek, David A; Maghsoodi, Bita; Borbon, Ivan A; Hardwick, Rhiannon N; Cherrington, Nathan J; Erickson, Robert P.
Afiliação
  • Jelinek DA; Dept of Pediatrics, Box 5073, University of Arizona Health Sciences Center, Tucson, AZ 85724-5073, USA. dajelinek@salud.unm.edu
Gene ; 491(2): 128-34, 2012 Jan 10.
Article em En | MEDLINE | ID: mdl-22020183
ABSTRACT
We have previously shown that male Npc1 heterozygous mice (Npc1(+/-)), as compared to homozygous wild-type mice (Npc1(+/+)), both maintained on the "lean" BALB/cJ genetic background, become obese on a high fat but not on a low fat diet. We have now extended this result for female heterozygous mice. When fed high-fat diet, the Npc1(+/-) white adipose weight is also increased in females, therefore following the same trend as males. Bile transporters which had previously been found to be altered in Npc1(-/-) mice on a high fat diet, showed related, but small, changes in mRNA levels but large changes in protein expression. We have addressed the possible role of caveolae in these differences. It has long been known that caveolin 1 is increased in the liver (sex not specified) of Npc1(+/-) (compared to Npc1(+/+) and Npc1(-/-)) mice and in heterozygous cultured skin fibroblasts of NPC1 carriers. We now find that caveolin 1 is increased in male, but not female liver and female, but not male adipose tissue. The caveolin 1 increase was not accompanied by changes in another caveolar protein, polymerase1 and transcript release factor (Ptrf). The numbers of caveolae in female adipose cells could not be correlated with levels of caveolae. Thus, we conclude that Npc1 affects female as well as male obesity and bile transporters but that effects on caveolin 1 are not discernible.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Bile / Doenças de Niemann-Pick / Cavéolas / Caveolina 1 / Adiposidade / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Gene Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Bile / Doenças de Niemann-Pick / Cavéolas / Caveolina 1 / Adiposidade / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Gene Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Estados Unidos