Improvement and validation of ¹²5I-high-performance liquid chromatography method for determination of total human serum choline and ethanolamine plasmalogens.
Ann Clin Biochem
; 49(Pt 1): 86-93, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22042981
ABSTRACT
BACKGROUND:
Serum plasmalogens (Pls) have gained interest in several clinical symptoms such as metabolic syndrome/atherosclerosis or Alzheimer's disease possibly because of their antioxidant properties. We have developed a highly sensitive and simple method to determine plasmenylcholine (PlsCho; choline plasmalogen) and plasmenylethanolamine (PlsEtn; ethanolamine plasmalogen) separately, using a radioactive iodine and high-performance liquid chromatography ((125)I-HPLC method). The present study reports the improvement and validation of (125)I-HPLC method by introducing a quantitative standard (QS) and online detection with a flow γ-counter.METHODS:
1-Alkenyl 2,3-cyclic glycerophosphate was prepared as QS from l-α-lyso plasmenylcholine by enzymatic treatment with phospholipase D. Online detection with a flow γ-counter was investigated to be available to quantify Pls. The method validation was carried out in terms of selectivity, sensitivity, linearity, precision, accuracy and recovery.RESULTS:
Linearity was established over the concentration range 5-300 µmol/L for Pls and QS with regression coefficients >0.99. The accuracy and reliability were satisfactory. The method has been applied to the determination of human serum Pls from healthy subjects and the elderly with dementia or artery stenoses.CONCLUSIONS:
The improved (125)I-HPLC method is useful as an autoanalytical system for a routine diagnostic test of human serum Pls.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmalogênios
/
Cromatografia Líquida de Alta Pressão
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Estenose Coronária
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Demência
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Aged
/
Aged80
/
Animals
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Female
/
Humans
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Male
Idioma:
En
Revista:
Ann Clin Biochem
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão