Compartmental approach for evaluation of plasma kinetics and (13)co(2)-exhalation after oral loading with L-[1-(13)c]leucine.
Isotopes Environ Health Stud
; 32(2-3): 237-46, 1996 Aug.
Article
em En
| MEDLINE
| ID: mdl-22088115
ABSTRACT
Abstract A seven compartment model was applied for evaluation of oral L-[1-(13)C]leucine loading tests (38 µmol/kg body wt.) in healthy volunteers. The model comprises transport and absorption in stomach and gut into a central L-leucine-compartment which is connected to a protein compartment and to the compartment of the corresponding 2-oxo acid. CO(2) release from the latter occurs in a fast and a slow compartment into the central CO(2) compartment for exhalation. Using the fmins routine of MATLAB for parameter estimation, a good agreement was obtained between calculated and actually measured kinetics of (13)C-labelled metabolites and a mean in vivo L-leucine oxidation of 0.365 ± 0.071 µmol/kg per min (n = 5) was computed. Plausibility of the model was checked by predicting in vivo leucine oxidation rates from primed continuous infusion tests (priming L-[1-(13)C]leucine, 5 µmol/kg; NaH(13)CO(2), 1.2 µmol/kg; infusion L-[1-(13)C]leucine, 5 µmol/kg per h). In 5 tested volunteers, the experimental L-leucine oxidation rate amounted to 0.358 ± 0.105 µmol/kg per min versus predicted 0.324±0.099 µmol/kg per min. Possible causes for some observed intraindividual variations are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Isotopes Environ Health Stud
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
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REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM