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Transepithelial transport of 4-chloro-2-methylphenoxyacetic acid (MCPA) across human intestinal Caco-2 cell monolayers.
Kimura, Osamu; Tsukagoshi, Kensuke; Hayasaka, Moriaki; Endo, Tetsuya.
Afiliação
  • Kimura O; Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Basic Clin Pharmacol Toxicol ; 110(6): 530-6, 2012 Jun.
Article em En | MEDLINE | ID: mdl-22181038
Mechanisms of transcellular transport of 4-chloro-2-methylphenoxyacetic acid (MCPA) across the small intestine were investigated using Caco-2 cells cultured on permeable membranes. The cell monolayers were incubated with MCPA, either from apical side at pH 6.0 or 7.4, or basolateral side at pH 7.4. The accumulation and apical-to-basolateral transport of MCPA were markedly stimulated by the acidic pH on the apical side (inwardly directed H(+) gradient), dependent on metabolic energy and inhibited by co-incubation with acetic acid or benzoic acid. Without the H(+) gradient, on the other hand, the basolateral-to-apical transport of MCPA (secretory transport) was higher than the apical-to-basolateral transport (absorptive transport), although the secretory transport of MCPA was markedly lower than the absorptive transport under the H(+) gradient. Co-incubation of MCPA with probenecid from the basolateral side significantly inhibited the accumulation and transport of MCPA, whereas co-incubation with p-aminohippuric acid did not. These results suggest that the absorptive transport of MCPA is mediated by H(+)-linked monocarboxylic acid transporters expressed on the apical membranes, while secretory transport is mediated by a probenecid-sensitive transporter expressed on the basolateral membranes of Caco-2 cell monolayers.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido 2-Metil-4-clorofenoxiacético / Absorção Intestinal / Intestino Delgado Limite: Humans Idioma: En Revista: Basic Clin Pharmacol Toxicol Assunto da revista: FARMACOLOGIA / TOXICOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido 2-Metil-4-clorofenoxiacético / Absorção Intestinal / Intestino Delgado Limite: Humans Idioma: En Revista: Basic Clin Pharmacol Toxicol Assunto da revista: FARMACOLOGIA / TOXICOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido