B lymphopoiesis is characterized by pre-B cell marker gene expression in fetal cattle and declines in adults.
Dev Comp Immunol
; 37(1): 39-49, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22210545
ABSTRACT
Fetal cattle B-cell development proceeds via a pre-B cell stage that is characterized by the expression of surrogate light chain and recombination activation genes. In this paper, we identify a new member of bovine pre-B lymphocyte genes, VPREB2. Using RT-qPCR, we assess the expression of VPREB2 and three other surrogate light chain genes as well as RAG1 and RAG2 in fetal and adult cattle tissues. The absence of VPREB1, IGLL1, RAG1 and RAG2 expression in adult tissues and the lack of B-lymphoid differentiation in adult bone marrow - OP9 stromal cell co-culture, suggest a decline of B lymphopoiesis in adult cattle. The marked differences in the expression profiles of VPREB2 and VPREB3 in comparison to those of VPREB1, IGLL1 and RAGs suggest that the biological roles of VPREB2 and VPREB3 are unrelated to the pre-B cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
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Expressão Gênica
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Linfopoese
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Cadeias Leves Substitutas da Imunoglobulina
/
Feto
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Finlândia