The protective effect of 17ß-estradiol against hydrogen peroxide-induced apoptosis on mesenchymal stem cell.
Biomed Pharmacother
; 66(1): 57-63, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-22281292
ABSTRACT
This study was designed to investigate the function of 17ß-estradiol (17ß-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2). BMSCs were treated with 17ß-E2 for 24h and then treated with 100µM H2O2 for 1h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H2O2 compared to control group. But pretreatment with 17ß-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17ß-E2 exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17ß-E2 can reduce the donor stem cells damage.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Estresse Oxidativo
/
Estradiol
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Biomed Pharmacother
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China