Neurons and stromal stem cells as targets for polycation-mediated transfection.
Bull Exp Biol Med
; 151(1): 126-9, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-22442817
ABSTRACT
Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Transfecção
/
Dendrímeros
/
Células-Tronco Mesenquimais
/
Neurônios
Limite:
Humans
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Canadá