Zoledronic acid influences growth, migration and invasive activity of prostate cancer cells in vitro.
Prostate Cancer Prostatic Dis
; 15(3): 250-5, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22450844
ABSTRACT
BACKGROUND:
The influence of the bisphosphonate zoledronic acid (ZA) on prostate cancer (PC) growth, adhesion and invasive behavior was investigated.METHODS:
PC-3, DU-145 and LNCaP cells were treated with ZA, and tumor-cell growth was then investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Furthermore, tumor-cell adhesion to vascular endothelium or to immobilized extracellular matrix proteins, as well as migratory properties of the cells, was evaluated. Integrin ß subtypes, integrin-dependent signaling, as well as cell-cycle regulating proteins, were analyzed by western blots.RESULTS:
ZA dose-dependently reduced tumor-cell growth but did not impair tumor-endothelium and tumor-matrix interaction. However, ZA significantly inhibited tumor migration and invasive activity. Cyclin E was reduced by ZA in LNCaP and DU-145, and p21 was elevated in LNCaP cells. p27 was upregulated in all tumor cell lines, compared with the controls. ZA elevated ß1-integrin in PC-3 and diminished ß4-integrin in PC-3 and DU-145 cells.CONCLUSIONS:
ZA inhibits PC growth and motility but does not influence the mechanical contact between tumor cells and the vascular wall.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Adesão Celular
/
Movimento Celular
/
Apoptose
/
Difosfonatos
/
Imidazóis
/
Antineoplásicos
Limite:
Humans
/
Male
Idioma:
En
Revista:
Prostate Cancer Prostatic Dis
Assunto da revista:
ENDOCRINOLOGIA
/
NEOPLASIAS
/
UROLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Alemanha