Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells.
Cell Stem Cell
; 10(4): 398-411, 2012 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-22482505
ABSTRACT
Two populations of Nkx2-1(+) progenitors in the developing foregut endoderm give rise to the entire postnatal lung and thyroid epithelium, but little is known about these cells because they are difficult to isolate in a pure form. We demonstrate here the purification and directed differentiation of primordial lung and thyroid progenitors derived from mouse embryonic stem cells (ESCs). Inhibition of TGFß and BMP signaling, followed by combinatorial stimulation of BMP and FGF signaling, can specify these cells efficiently from definitive endodermal precursors. When derived using Nkx2-1(GFP) knockin reporter ESCs, these progenitors can be purified for expansion in culture and have a transcriptome that overlaps with developing lung epithelium. Upon induction, they can express a broad repertoire of markers indicative of lung and thyroid lineages and can recellularize a 3D lung tissue scaffold. Thus, we have derived a pure population of progenitors able to recapitulate the developmental milestones of lung/thyroid development.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândula Tireoide
/
Separação Celular
/
Células-Tronco Embrionárias
/
Pulmão
Limite:
Animals
Idioma:
En
Revista:
Cell Stem Cell
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos