A monoclonal antibody SZ-117 that recognizes filamin A derived from tumor cells.
Hybridoma (Larchmt)
; 31(3): 214-8, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22741587
ABSTRACT
SZ117 is a monoclonal antibody against matrix metalloproteinase-2 (MMP-2) and exhibits anti-tumor angiogenic effect. In this study, we observed that SZ117 bound to a 280 kDa protein, which was detected in tumor cell-derived Matrigel and various tumor cells. Using immunoprecipitation, mass spectrometry analysis, and Western blot analysis, we identified the 280 kDa protein as filamin A and found that filamin A and its degraded products, notably a 53 kDa fragment, were released from a variety of tumor cells. This suggests that SZ117 is useful in the study of the pathogenesis of filamin A and that blockage of filamin A by SZ117 might contribute to the anti-tumor angiogenic effect of the monoclonal antibody.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas Contráteis
/
Proteínas dos Microfilamentos
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Revista:
Hybridoma (Larchmt)
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China