Short chain phospholipids in membrane protein crystallization: a 31P-NMR study of colloidal properties of dihexanoyl phosphatidylcholine.
Chem Phys Lipids
; 55(3): 351-4, 1990 Sep.
Article
em En
| MEDLINE
| ID: mdl-2289291
ABSTRACT
The colloidal features of short chain phospholipids can be deduced from 31P-NMR analysis by comparison with available data on phospholipid aqueous dispersion. In this study with dihexanoyl phosphatidylcholine, detergent phase separation was obtained by temperature shift and by addition of the precipitating agent polyethylene glycol. The 31P-NMR spectra indicate that the detergent micelles fuse to enter the hexagonal HII and lamellar phases. Consequences for the crystallization of membrane proteins are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Proteínas de Membrana
Idioma:
En
Revista:
Chem Phys Lipids
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
França
País de publicação:
IE
/
IRELAND
/
IRLANDA