Intracellular Ca2+ and antioxidant values induced positive effect on fertilisation ratio and oocyte quality of granulosa cells in patients undergoing in vitro fertilisation.
Reprod Fertil Dev
; 25(5): 746-52, 2013.
Article
em En
| MEDLINE
| ID: mdl-22954014
ABSTRACT
Oxidative stress is important for promoting oocyte maturation and ovulation within the follicle through calcium ion (Ca(2+)) influx. The relationship between antioxidant and cytosolic Ca(2+) levels and oocyte quality and fertilisation rate in the granulosa cells of patients undergoing in vitro fertilisation was investigated. Granulosa cells were collected from 33 patients. Cytosolic free Ca(2+) ([Ca(2+)]i) concentration, lipid peroxidation, reduced glutathione, glutathione peroxidase and oocyte quality were measured in the granulosa cells. The relationship between two drug protocols was also examined (gonadotrophin-releasing hormone antagonist and agonist protocols) and the same parameters investigated. The [Ca(2+)]i concentration (P<0.001), glutathione (P<0.05) and oocyte quality (P<0.001) values were significantly higher in the fertilised group than in the non-fertilised group, although glutathione peroxidase activity was significantly (P<0.05) higher in the non-fertilised group than in the fertilised group. The [Ca(2+)]i concentrations were also higher (P<0.001) in the good-quality oocyte groups than in the poor-quality oocyte group. There was no correlation between the two drug protocols and investigated parameters. In conclusion, it was observed that high glutathione and cytosolic Ca(2+) concentrations in granulosa cells of patients undergoing in vitro fertilisation tended to increase the fertilisation potential of oocytes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Fertilização in vitro
/
Cálcio
/
Sinalização do Cálcio
/
Fertilização
/
Células da Granulosa
/
Antioxidantes
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Reprod Fertil Dev
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Turquia