Dioscin: a synergistic tyrosinase inhibitor from the roots of Smilax china.
Food Chem
; 134(2): 1146-8, 2012 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-23107741
ABSTRACT
In our study, we investigated the inhibition of mushroom tyrosinase in Smilax china. A methanol (MeOH) extract of S. china was partitioned into hexane, ethyl acetate (EtOAc) and water. Of the three fractions, EtOAc extract showed the strongest inhibition of tyrosinase activity with l-tyrosine or l-DOPA as a substrate. Two compounds were isolated from a final active fraction by activity-guided column chromatography. These compounds were identified as dioscin and oxyresveratrol by comparing their mass, (1)H- and (13)C-NMR spectral data with those reported in the literature. Dioscin showed little inhibition activity of tyrosinase, whereas oxyresveratrol, a known tyrosinase inhibitor, showed a strong tyrosinase inhibitory activity. We discovered that a mixture of oxyresveratrol and dioscin (IC(50)=5.1 and 5.7 µg/ml) highly increased the inhibition of tyrosinase activity with l-tyrosine or l-DOPA as the substrate as compared to either oxyresveratrol (IC(50)=7.8 and 10.9 µg/ml) or dioscin (IC(50)>100 and 100 µg/ml) alone.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
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Extratos Vegetais
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Monofenol Mono-Oxigenase
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Agaricales
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Smilax
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Diosgenina
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Inibidores Enzimáticos
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2012
Tipo de documento:
Article