Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells.
Food Chem
; 136(2): 322-7, 2013 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-23122065
ABSTRACT
The inhibitory effect of an aqueous extract from spinach on degranulation of RBL-2H3 cells is herein reported. The extract significantly suppressed antigen-induced degranulation in a dose-dependent manner without affecting cell viability. Active substances in the extract were heat-stable and trypsin-resistant with molecular weights ranging from 500 Da to 14 kDa. The extract inhibited elevation of the intracellular Ca(2+) concentration caused by stimulation by antigen, while not suppressing degranulation induced by a calcium ionophore A23187. Immunoblot analysis revealed that the inhibitory effect results from downregulation of phosphorylation of both Syk kinase and phosphatidylinositol 3-kinase in the signalling pathways involved in degranulation caused by the antigen-antibody interaction. Taken together, these findings suggest that aqueous spinach extract has an anti-allergic activity that controls degranulation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Basófilos
/
Extratos Vegetais
/
Degranulação Celular
/
Spinacia oleracea
/
Antialérgicos
Limite:
Animals
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão