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Genotoxicity assessment of amaranth and allura red using Saccharomyces cerevisiae.
Jabeen, Hafiza Sumara; ur Rahman, Sajjad; Mahmood, Shahid; Anwer, Sadaf.
Afiliação
  • Jabeen HS; Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan. Sumara.jabeen@gmail.com
Bull Environ Contam Toxicol ; 90(1): 22-6, 2013 Jan.
Article em En | MEDLINE | ID: mdl-23132362
Amaranth (E123) and Allura red (E129), very important food azo dyes used in food, drug, paper, cosmetic and textile industries, were assessed for their genotoxic potential through comet assay in yeast cells. Comet assay was standardized by with different concentration of H(2)O(2). Concentrations of Amaranth and Allura red were maintained in sorbitol buffer starting from 9.76 to 5,000 µg/mL and 1 × 10(4) cells were incubated at two different incubation temperatures 28 and 37°C. Amaranth (E123) and Allura red (E129) were found to exhibit their genotoxic effect directly in Saccharomyces cerevisiae. No significant genotoxic activity was observed for Amaranth and Allura red at 28°C but at 37°C direct relation of Amaranth concentration with comet tail was significant and no positive relation was seen with time exposure factor. At 37°C the minimum concentration of Amaranth and Allura red at which significant DNA damage observed through comet assay was 1,250 µg/mL in 2nd h post exposure time. The results indicated that food colors should be carefully used in baking products as heavy concentration of food colors could affect the fermentation process of baking.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Compostos Azo / Corantes / Poluentes Ambientais / Corante Amaranto Idioma: En Revista: Bull Environ Contam Toxicol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Compostos Azo / Corantes / Poluentes Ambientais / Corante Amaranto Idioma: En Revista: Bull Environ Contam Toxicol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Estados Unidos