Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold.
J Orthop Surg Res
; 7: 40, 2012 Dec 19.
Article
em En
| MEDLINE
| ID: mdl-23253362
ABSTRACT
BACKGROUND:
Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done.METHODS:
We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR.RESULTS:
The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels.CONCLUSIONS:
This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Condrócitos
/
Condrogênese
/
Alicerces Teciduais
/
Gelatina
/
Ácido Hialurônico
Limite:
Adolescent
/
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Orthop Surg Res
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Tailândia