Ght2⺠is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.
Appl Microbiol Biotechnol
; 97(11): 4957-64, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23254763
Schizosaccharomyces pombe has eight hexose transporter genes, ght1 (+) to ght8 (+). Here we report that ght2 (+), which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 (+) in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Uridina Difosfato Galactose
/
Proteínas de Transporte de Monossacarídeos
/
Galactose
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
Alemanha