Stimulation of glycoprotein and protein synthesis in isolated pig gastric mucosal cells by prostaglandins.
Gut
; 31(4): 412-6, 1990 Apr.
Article
em En
| MEDLINE
| ID: mdl-2338265
ABSTRACT
The purpose of this study is to evaluate the effects of different prostaglandin derivatives on protein and glycoprotein synthesis and secretion in isolated and enriched pig gastric mucous cells, as measured by the incorporation of [3H]L-leucine and N-acetyl-[14C]D-glucosamine respectively into acid insoluble macromolecules (AIM). PGE2 and 16,16-dimethyl-PGE2 enhanced the incorporation of the amino sugar into cellular (EC50 8 and 75 nmol/l) and secreted (EC50 30 and 270 nmol/l) AIM in a concentration dependent manner during a 20 hours incubation. After incubation for eight hours or more they also stimulated the incorporation of [3H]L-leucine into cellular AIM. PGF2 alpha was considerably less potent (EC50 greater than 1 mumol/l) than the E-type prostaglandins. Iloprost, a stable prostacyclin analogue, was ineffective.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prostaglandinas E Sintéticas
/
Biossíntese de Proteínas
/
Dinoprosta
/
Dinoprostona
/
16,16-Dimetilprostaglandina E2
/
Mucosa Gástrica
Limite:
Animals
Idioma:
En
Revista:
Gut
Ano de publicação:
1990
Tipo de documento:
Article