Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.
Methods Enzymol
; 525: 265-84, 2013.
Article
em En
| MEDLINE
| ID: mdl-23522474
ABSTRACT
The ciliate Tetrahymena thermophila is an excellent model system for the discovery and functional studies of ciliary proteins. The power of the model is based on the ease with which cilia can be purified in large quantities for fractionation and proteomic identification, and the ability to knock out any gene by homologous DNA recombination. Here, we include methods used by our laboratories for isolation and fractionation of cilia, in vivo tagging and localization of ciliary proteins, and the evaluation of ciliary mutants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cílios
/
Tetrahymena thermophila
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos