Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model.
Biotechnol Lett
; 35(8): 1191-7, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23553521
ABSTRACT
Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 µg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte de Cátions
/
Hepcidinas
/
Ferro
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Irã