Priming ammonia lyases and aminomutases for industrial and therapeutic applications.
Curr Opin Chem Biol
; 17(2): 250-60, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23557642
ABSTRACT
Ammonia lyases (AL) and aminomutases (AM) are emerging in green synthetic routes to chiral amines and an AL is being explored as an enzyme therapeutic for treating phenylketonuria and cancer. Although the restricted substrate range of the wild-type enzymes limits their widespread application, the non-reliance on external cofactors and direct functionalization of an olefinic bond make ammonia lyases attractive biocatalysts for use in the synthesis of natural and non-natural amino acids, including ß-amino acids. The approach of combining structure-guided enzyme engineering with efficient mutant library screening has extended the synthetic scope of these enzymes in recent years and has resolved important mechanistic issues for AMs and ALs, including those containing the MIO (4-methylideneimidazole-5-one) internal cofactor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia Industrial
/
Engenharia de Proteínas
/
Transferases Intramoleculares
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Indústria Farmacêutica
/
Amônia-Liases
Idioma:
En
Revista:
Curr Opin Chem Biol
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Holanda