Glutathione-triggered formation of molecular hydrogels for 3D cell culture.
Colloids Surf B Biointerfaces
; 108: 352-7, 2013 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-23587765
ABSTRACT
The development of three dimensional (3D) scaffolds that are suitable for cell encapsulation and proliferation is highly important for tissue engineering and regenerative medicine. We reported in this paper on several molecular hydrogels formed through glutathione (GSH) reduction, whose mechanical property and zeta potential could be regulated by concentration and structure of gelators in resulting gels, respectively. The hydrogels were characterized by several techniques including rheology, TEM and fluorescence. We found that, in our system, the mechanical property of hydrogels but not the zeta potential of self-assembled structures had big influences on mouse fibroblast 3T3 cells spreading and proliferation. Hydrogels with storage modulus (G') of hundreds of pascals (Pa) were suitable for 3T3 cells spreading and proliferation. We believed that hydrogels reported in this study had big potential for applications in different fields, such as 3D cell culture and tissue engineering.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Materiais Biocompatíveis
/
Técnicas de Cultura de Células
/
Glutationa
Limite:
Animals
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS