CENP-A confers a reduction in height on octameric nucleosomes.

Miell, Matthew D D; Fuller, Colin J; Guse, Annika; Barysz, Helena M; Downes, Andrew; Owen-Hughes, Tom; Rappsilber, Juri; Straight, Aaron F; Allshire, Robin C

Miell, Matthew D D; Fuller, Colin J; Guse, Annika; Barysz, Helena M; Downes, Andrew; Owen-Hughes, Tom; Rappsilber, Juri; Straight, Aaron F; Allshire, Robin C.

Afiliação

Miell MD; Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Nat Struct Mol Biol ; 20(6): 763-5, 2013 Jun.

Article em En | MEDLINE | ID: mdl-23644598

ABSTRACT

ABSTRACT

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

Assuntos

Autoantígenos/metabolismo; Proteínas Cromossômicas não Histona/metabolismo; Nucleossomos/metabolismo; Nucleossomos/ultraestrutura; Proteína Centromérica A; Humanos; Microscopia de Força Atômica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autoantígenos / Proteínas Cromossômicas não Histona / Nucleossomos Limite: Humans Idioma: En Revista: Nat Struct Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Reino Unido

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