Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease.
Anim Reprod Sci
; 140(1-2): 14-25, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23693122
ABSTRACT
Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P<0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cistos Ovarianos
/
Fator de Crescimento Insulin-Like I
/
Bovinos
/
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina
Limite:
Animals
Idioma:
En
Revista:
Anim Reprod Sci
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Argentina