Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus.
Int J Biol Macromol
; 60: 93-9, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23707733
ABSTRACT
Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and destruction of motor neurons. This work evaluated the activity of a sulfated polysaccharide of Caesalpinia ferrea (SPLCf) in HSV and PV replication. The antiviral effect of SPLCf at varying concentrations was tested by plaque assay under several protocols, such as time-of-addition, adsorption and penetration inhibition and virucidal. Syntheses of viral protein and nucleic acid were also monitored by the immunofluorescence assay and PCR. The SPLCf inhibited virus adsorption and steps after penetration, and inhibited the synthesis of viral protein. Virucidal effect was also shown and nucleic acid synthesis was concurrent with positive results. Our findings suggested that the substance with low toxicity represent a potential viral inhibitor.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Polissacarídeos
/
Extratos Vegetais
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Simplexvirus
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Caesalpinia
/
Poliovirus
Limite:
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil