Remineralization of demineralized bone matrix (DBM) via alternating solution immersion (ASI).
J Mech Behav Biomed Mater
; 26: 109-18, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23759125
ABSTRACT
In order to achieve successful clinical outcomes, biomaterials used for bone grafts must possess a number of traits including biocompatibility and osteoconductivity. These materials must also demonstrate appropriate mechanical stability to withstand handling as well as support potentially significant stresses at the implant site. Synthetic and natural polymer scaffolds used for bone tissue engineering (BTE) often lack necessary mechanical properties. Our goal was to internally mineralize natural collagenous matrix, thereby increasing mechanical properties of the material to useful levels. Published methods for intrafibrillar collagen mineralization were applied to clinically relevant-sized constructs but did not successfully deposit mineral in the interior of the constructs. To address this limitation, we developed a new technique for the remineralization of demineralized bone matrix (DBM) based on alternating solution immersion, or ASI. Mineral was removed from equine bone specimens, leaving behind a demineralized bone matrix (DBM). This matrix provides a framework for the nucleation and growth of a replacement mineral phase. Plain film radiography and microcomputed tomography (microCT) indicated accumulation of mineral within the DBM, and mechanical testing (3 point bending and compression) revealed a significant increase in stiffness between the DBM and the remineralized bone matrix (RBM). We believe this remineralization process will be useful in the preparation of stiff and strong allografts for clinical application.
Palavras-chave
ASI; BTE; Bone graft; Collagen; DBM; HA; MC3; MVF; Matrix; Mineralization; RBM; SDS; Tissue engineering; alternating solution immersion; bone tissue engineering; demineralized bone matrix; hydroxyapatite; mineral volume fraction; remineralized bone matrix; sodium dodecyl sulfate; third metacarpal bone
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Matriz Óssea
/
Calcificação Fisiológica
/
Imersão
Limite:
Animals
Idioma:
En
Revista:
J Mech Behav Biomed Mater
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos