Interaction of acriflavine with pyrimidines: a spectroscopic approach.
Spectrochim Acta A Mol Biomol Spectrosc
; 114: 316-22, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23778172
ABSTRACT
The interaction of acriflavine with uracils was investigated by using spectroscopic tools viz., UV-Vis absorption, steady state and time resolved fluorescence measurements. The spectroscopic data were analyzed using Stern-Volmer equation to determine the quenching process. The bimolecular quenching rate constant (k(q)), binding constant (K) and number of binding sites (n) were calculated at different temperature from the relevant fluorescence data. The experimental results obtained from life-time measurement indicate that the quenching mechanism was static via the formation of ground state complex. The free energy change (ΔGet) for electron transfer process was calculated by Rehm-Weller equation. The existence of binding forces and the interactions of acriflavine with uracils were examined.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uracila
/
Acriflavina
/
Fármacos Fotossensibilizantes
/
Corantes Fluorescentes
/
Anti-Infecciosos Locais
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Índia