Impaired lipid accumulation in the liver of Tsc2-heterozygous mice during liver regeneration.
Biochem Biophys Res Commun
; 437(1): 146-50, 2013 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-23810393
ABSTRACT
Tuberin is a negative regulator of mTOR pathway. To investigate the function of tuberin during liver regeneration, we performed 70% hepatectomy on wild-type and Tsc2+/- mice. We found the tuberin phosphorylation correlated with mTOR activation during early liver regeneration in wild-type mice. However, liver regeneration in the Tsc2+/- mice was not enhanced. Instead, the Tsc2+/- livers failed to accumulate lipid bodies, and this was accompanied by increased mortality. These findings suggest that tuberin plays a critical role in liver energy balance by regulating hepatocellular lipid accumulation during early liver regeneration. These effects may influence the role of mTORC1 on cell growth and proliferation.
Palavras-chave
4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride; 5'AMP-activated protein kinase; AEBSF; AMPK; C57BL/6; ERK; GAP; GTPase-activating protein; Lipid; Liver regeneration; MAPK; P70S6K; PH; Rheb; SH; TSC; TSC2; Tuberin; c57 black 6; extracellular signal-regulated kinase; mTOR; mammalian target of rapamycin; mitogen-activated protein kinase; p70 ribosomal S6 kinase; p90 RSK; p90 ribosomal S6 kinase; partial hepatectomy; ras homolog enriched in brain; sham operation; tuberous sclerosis
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Supressoras de Tumor
/
Metabolismo dos Lipídeos
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Heterozigoto
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Fígado
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Regeneração Hepática
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos