Enzymatic assay for Cu(II) with horseradish peroxidase and its application in colorimetric logic gate.
Anal Chem
; 85(15): 7029-32, 2013 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-23879883
ABSTRACT
We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Cobre
/
Peroxidase do Rábano Silvestre
/
Lógica
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2013
Tipo de documento:
Article