[The apoptosis effect of antisense oligodeoxynucleotides targeting ATM on the laryngeal squamous cell carcinoma treated with radiation].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 44(3): 379-82, 2013 May.
Article
em Zh
| MEDLINE
| ID: mdl-23898517
ABSTRACT
OBJECTIVE:
To designed antisense oligodeoxynucleotides (AS-ODNs) to reduce the expression of ATM and to study its effect on the apoptosis of Hep-2 (human epidermoid laryngeal carcinoma) cells treated with radiation in vitro.METHODS:
Experiment was divided into AS-Lipo, Sen-Lipo, Mis-Lipo, Lipo and Hep-2 group. The expression of ATM mRNA in Hep-2 cells was examined by real-time quantitative PCR. About 18 hours after transfection, they were irradiated simultaneously with different doses of X-ray radiation (0, 2, 4, 6, and 8 Gy) respectively. Clonogenic survival assay was carried out to detect the survival ability of Hep-2 cells after irradiation. After exposed to 4 Gy radiation, flow cytometry was carried out to analyze the cell apoptosis.RESULTS:
The relative ATM mRNA expression in Hep-2 cells treated with ATM AS-ODNs was decreased to (11.03 +/- 2.51)% which was much lower than that of untreated cells (P < 0.05). After irradiation, the survival fraction (SF) of cells treated with ATM AS-ODNs was lower than that of other groups at the same dose of radiation. There was statistical significance between the group treated with ATM AS-ODNs and other groups (P < 0.05). The apoptotic rate for the group irradiated with ATM AS-ODNs was (30.7 +/- 1.31)%, which was significantly higher than that of others (P < 0.05).CONCLUSION:
AS-ODNs of ATM reduce ATM mRNA expression and enhance Hep-2 cells apoptosis to radiation in vitro.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Escamosas
/
Neoplasias Laríngeas
/
Apoptose
/
Oligodesoxirribonucleotídeos Antissenso
/
Proteínas Mutadas de Ataxia Telangiectasia
Limite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China