Sequencing and characterization of the Monocellicampa pruni (Hymenoptera: Tenthredinidae) mitochondrial genome.

ABSTRACT

The mitochondrial genome of the Monocellicampa pruni (Hymenoptera Tenthredinidae) (GenBank accession No. JX566509) has been reported in this study. This is the first sequenced mitochondrial genome from the family Tenthredinidae of the order Hymenoptera. The sequenced region of this mitochondrial genome is 15,169 bp with an A + T content of 77.21%, including 13 protein-coding, 2 rRNA and 19 tRNA genes, and a partial region of the A + T-rich region. Three tRNA genes, i.e. trnI. trnQ and trnM, between the A + T-rich region and the nad2 gene were failed to sequence because of the present of PolyAT structure. The gene arrangement of the sequenced region was similar to the pupative ancestral arrangement of insects. There are two large non-coding regions located between trnC and trnY. trnF and nad5 with a length of 107 and 177 bp, respectively. All protein-coding genes start with ATN start codon. Eleven protein-coding genes stop with termination codon TAA, whereas one protein-coding gene uses incomplete stop codon TA and one uses T. All of the 22 tRNA genes have a typical cloverleaf structure except for the trnS1, in which, the D-stem pairings in the DHU arm are absent.