Development and validation of a high-performance liquid chromatography ultraviolet method for lapatinib quantification in human plasma.
Ther Drug Monit
; 35(6): 796-802, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-23942544
ABSTRACT
A selective and precise high-performance liquid chromatography ultraviolet method was developed and validated for the determination of lapatinib in human plasma. After protein precipitation with acetonitrile, lapatinib and sorafenib were separated using isocratic elution (on a C18 Ultrabase column using a mobile phase of acetonitrile/20 mM ammonium acetate in a proportion 5347 (v/v) pumped at a constant flow rate of 1.2 mL/min). Quantification was performed at 260 nm. Calibration curves were linear over the range 0.2-10 µg/mL. Inter- and intraday coefficients of variation were less than 7%. The limit of detection and the lower limit of quantification were 0.1 and 0.2 µg/mL, respectively. Recoveries of lapatinib from plasma were higher than 86.7% in all cases. The assay was applied to the determination of the drug in the plasma of 2 cancer patients receiving lapatinib, 1000 and 1250 mg orally, and could be useful for therapeutic drug monitoring of lapatinib in routine clinical practice.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinazolinas
/
Cromatografia Líquida de Alta Pressão
/
Monitoramento de Medicamentos
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Ther Drug Monit
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Espanha