An optimized device for staining electron microscopy grids.
Biotech Histochem
; 89(1): 66-74, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-23962218
ABSTRACT
Proper staining of grids is critical for transmission electron microscopy (TEM). Staining must be done as quickly as possible using minimal reagents and with consideration for the environment. We developed a new device for efficient staining of multiple TEM grids. We studied reagent evaporation, rinsing volume, flow rate and re-use of uranyl acetate, and provide here a procedure for efficient staining using the new device. Our device permits TEM grids to be stained with less reagent than alternative staining apparatuses; staining requires a total volume of 260 µl for five grids. Reagent evaporation is less than 6% even if used at 37° C. Moreover, our staining apparatus reduces chemical waste and shortens experiment time by staining several grids simultaneously. Our staining device is a compromise between time-consuming single grid processing and expensive commercial devices that consume large amounts of reagents.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Microscopia Eletrônica de Transmissão
Limite:
Humans
Idioma:
En
Revista:
Biotech Histochem
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
França
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM